Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

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Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+).It is now possible to hbl5266ca acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers.In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse.Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable.TOF-SIMS imaging shows the ability to localize directly on Sneakers for Men - Grey - Canvas Mesh Athletic Running Shoes a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.